Fig 1: Overexpression of miR-206 suppresses cisplatin resistance of resistant GC cells. The expression of miR-206 (a), IC50 of cisplatin (b), P-gp protein level (c and d) and apoptosis (e and f) were detected in MKN-7/DDP and HGC-27/DDP cells with transfection of miR-206 mimic or miR-NC via RT-qPCR, MTT, Western blot and flow cytometry, respectively. *P < 0.05.
Fig 2: lncRNA-H19 attenuated miR-29b-3p-mediated carboplatin sensitivity in EOC cells. SKOV3 and SKOV3-CB cells were transfected with miR-NC, miR-29b-3p, miR-29b-3p+pcDNA3.1, or miR-29b-3p+pcDNA3.1-H19, followed by detection of colony number (A, 200×), cell proliferation (B and C), migration (D, 200×), invasion (E, 200×), and the drug-resistance-related proteins P-gp, MRP1, and LRP (F and G). *P<0.05. IC50, 50% inhibitory concentration. For Figure 5F, lane 1-4 stands for miR-NC, miR-29b-3p, miR-29b-3p + pcDNA3.1, and miR-29b-3p + pcDNA3.1-H19, respectively.
Fig 3: Knockdown of CD133 expression in drug-resistant cell lines enhances cell sensitivity to chemotherapy drugs. (A) RT-qPCR was performed to detect the expression of CD133. RT-qPCR detected the expression of drug-resistant associated genes in (B) KOA-1/5-FU and (C) KOA-1/PYM cells. ***P<0.001. CD, cluster of differentiation; RT-qPCR, reverse transcription-quantitative PCR; 5-FU, 5-fluorouracil; PYM, pingyangmycin; siRNA, small interfering RNA; NC, negative control; MDR1, multidrug resistance protein 1; MRP1, multidrug resistance-associated protein 1.
Fig 4: Knockdown of CD133 expression in drug-resistant cell lines enhances cell sensitivity to chemotherapy drugs. (A and B) The expression levels of multi-drug resistance proteins MDR1 and MRP1 were detected by immunohistochemistry. CD, cluster of differentiation; MDR, multidrug resistance protein; MRP, multidrug resistance-associated protein; 5-FU, 5-fluorouracil; PYM, pingyangmycin; siRNA, small interfering RNA; NC, negative control.
Fig 5: Silence of circ_AKT3 inhibits cisplatin resistance of resistant GC cells. MKN-7/DDP and HGC-27/DDP cells were transfected with si-circ_AKT3 or s-NC. Then, circ_AKT3 expression (a), IC50 of cisplatin (b), P-gp protein level (c and d) and apoptosis (e and f) were measured via RT-qPCR, MTT, Western blot and flow cytometry, respectively. *P < 0.05.
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