Fig 1: NEK2 activated noncanonical NF-?B signaling pathway by phosphorylating NIK.A GST pull-down assays were used to explore the interaction between NEK2 and NIK. B and C Immunoblotting (IB) for IP using a NEK2 antibody (B) or a NIK antibody (C). Nonspecific antibody IgG was used as a negative control. D Western-blot assays were used to detect the phosphorylation levels of NEK2 knockdown GBM cells and control GBM cells. E Western-blot assays for measuring the protein levels of NIK in NEK2 knockdown GBM cells and control GBM cells. F qRT-PCR analysis was performed to detect the mRNA levels of NIK in NEK2 knockdown cells and control cells. G The effect of proteasome inhibitor MG132 on protein level of NIK in GBM cells pretreated with or without shNEK2 lentivirus. ß-actin was used as an internal control. All data were presented as the mean ± SD of triplicate independent experiments.