Fig 1: Expression of immune checkpoint antigens on BCO#147 organoids. Organoids of BCO#147, a population with limited in vitro growth capacity, were fixed and directly stained in early passage (left panel) or in later passage (right panel) cultures with antibodies reactive with PD-L1, CD276, and CD47 as indicated. For each staining, an overview with several organoids and a detailed picture of individual organoids are presented. Size bars indicate 200 µm and 20 µm, respectively.
Fig 2: Expression of immune checkpoint antigens on BCO#140. Paraffin sections of organoids of BCO#140, a population with high in vitro growth capacity, were generated and stained in early passage (left panel) or in later passage (right panel) with antibodies reactive with PD-L1, CD276, and CD47 as indicated. In the bottom panel, CD47 is visualized by red fluorescence, CD276 by green fluorescence. Size bars indicate 20 µm, respectively.
Fig 3: Expression of immune checkpoint antigens on BCO#154. Paraffin sections of organoids of BCO#154, a population with moderate in vitro growth capacity, were generated and stained in early passage (left panel) or in later passage (right panel) with antibodies reactive with PD-L1, CD276, and CD47 as indicated. In the bottom panel, CD47 is visualized by red fluorescence, CD276 by green fluorescence. Size bars indicate 20 µm, respectively.
Fig 4: Expression of immune checkpoint antigens on BCO#154 organoids. Organoids of BCO#154, a population with moderate in vitro growth capacity, were fixed and directly stained in early passage (left panel) or in later passage (right panel) cultures with antibodies reactive with PD-L1, CD276, and CD47 as indicated. For each staining, an overview with several organoids and a detailed picture of individual organoids are presented. Size bars indicate 200 µm, 100 µm, 20 µm, and 10 µm as indicated.
Fig 5: Expression of immune checkpoint antigens on BCO#147. Paraffin sections of organoids of BCO#147, a population with limited in vitro growth capacity, were generated and stained in early passage (left panel) or in later passage (right panel) with antibodies reactive with PD-L1, CD276, and CD47 as indicated. In the bottom panel, CD47 is visualized by red fluorescence, CD276 by green fluorescence. Size bars indicate 20 µm, respectively.
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