Fig 1: YAP m5C modification also relies on ALYREF and increases YAP mRNA stability.A The enrichment of ALYREF and YBX1 in A549 and H1299 cells at P1 region of YAP 3' UTR was calculated as the percentage of the input RNA via RNA-IP using the anti-ALYREF and anti-YBX1 antibodies. B YAP mRNA level was measured by qPCR in A549 and H1299 cells with ALYREF or YBX1 overexpressed with or without NSUN2 knockdown. C–D YAP protein level was measured by IB in A549 (C) and H1299 (D) cells with ALYREF or YBX1 overexpressed with or without NSUN2 knockdown. The level of proteins was normalized to that of GAPDH, and the normalized level of proteins in negative control groups were arbitrarily set to 1. E–J YAP mRNA stability was measured in A549 and H1299 cells with NUSN2 (E–F), ALYREF (G–H) and EXOSC10 (I–J) overexpressed or knocked down at indicated time after ActD treatment. The data are shown as the means ± SD from three independent experiments. Images of IB are representative ones of three independent experiments. *p < 0.05, **p < 0.01 indicate statistical significance. The data from (A–B) were analyzed by a one-way ANOVA test. The data from (E–J) were analyzed by a two-way ANOVA test.