Fig 2: Immunolocalization of GSTP1 in pig sperm. (A,B) Control-0h, semen samples at 0 h of storage. (C,D) Control-72h, vehicle control (dimethyl sulfoxide; DMSO) semen samples at 72 h of storage at 17°C. (E,F) TER-72h, semen samples treated with 100 μM ezatiostat (TER) for 72 h of storage at 17°C. (G,H) Negative control. The nucleus is shown in blue (DAPI), whereas GSTP1 is shown in green. Scale bars: (A,C,E): 30 μm; (B,D,F): 15 μm; (G): 20 μm; (H): 25 μm. These results are representative of three independent experiments (n = 3).

Fig 3: Immunoblotting analysis of GSTP1. Pig sperm lysates were incubated with (A) anti-GSTP1 antibody, (B) anti-α-tubulin antibody, and (C) total protein bands. Lanes: MW (kDa), molecular weight; Control-0h, semen samples at 0 h of storage; Control-72h, vehicle control (dimethyl sulfoxide; DMSO) semen samples at 72 h of storage at 17°C; TER-72h, semen samples treated with 100 μM ezatiostat (TER) for 72 h of storage at 17°C. Alpha-tubulin (anti-tubulin) and TGX Stain-FreeTM (total protein) were performed as complementary loading controls. These results are representative of three independent experiments (n = 3).