Fig 2: Impaired prepulse inhibition (PPI) in PlxnA1 KO mice. (A) The amplitude of the auditory startle reflex did not differ between PlxnA1 KO and WT mice. (B) %PPI was significantly reduced in PlxnA1 KO mice at a prepulse intensity of 86 dB compared to WT mice. (C) PlxnA1 KO-CC mice displayed significantly reduced %PPI at both 78 dB and 86 dB prepulse intensities compared to WT mice (C). WT (n = 34); PlxnA1 KO (n = 30); PlxnA1 KO-CC (n = 26); PlxnA1 KO-AgCC (n = 4). Data are presented as mean ± SEM. WT: wild type, KO: PlxnA1 knockout mice, PlxnA1 KO-CC: PlxnA1 KO mice with corpus callosum, PlxnA1 KO-AgCC: PlxnA1 KO mice with agenesis of corpus callosum.

Fig 3: Normal social behavior of PlxnA1 KO mice. (A, B) Both WT and PlxnA1 KO mice demonstrated a significant preference for the cage containing a stranger mouse (1) compared to an empty cage. (C, D) Neither genotype demonstrated a significant preference for the cage containing a novel mouse (stranger 2) compared to stranger 1. WT (n = 14); PlxnA1 KO (n = 14). Data are presented as mean ± SEM. WT: wild type, KO: PlxnA1 knockout mice.

Fig 4: Reduced anxiety-like behavior by PlxnA1 KO mice in the elevated plus maze. (A-E) PlxnA1 KO mice spent significantly more time on the open arms (B), a significantly higher percentage (%) of time on the open arms (C), significantly fewer closed arm entries (D), and significantly less time on the closed arms than WT mice (E). (F) KO mice also made a significantly lower percentage (%) of time on the closed arms than WT mice. WT (n = 14); PlxnA1 KO (n = 14). Data are presented as mean ± SEM. WT: wild type, KO: PlxnA1 knockout mice.

Fig 5: Normal working and fear memory in PlxnA1 K O mice. (A, B) Percentage alternations in the Y maze test (A) and number of arm entries (B) by WT and PlxnA1 KO mice. (C, D) No significant difference in freezing time by PlxnA1 KO mice compared to WT mice in both the contextual fear conditioning test (C) and cued fear conditioning test (D). WT (n = 14); PlxnA1 KO (n = 14). Data are presented as mean ± SEM. WT: wild type, KO: PlxnA1 knockout mice.