Anti-LAMP1 antibody GTX19294 from GeneTex

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Nature communications
March 07 2019

Fig 1: Therapeutic enhancement of lysosomal proteostasis in NM-producing rats. a Ipsilateral SNpc sections from rats co-injected with AAV-hTyr/flagged-AAV-TFEB immunostained with Flag (blue, left) or hTyr (blue, right) at 5 m post-AAV. NM, brown. Arrowheads, nuclear Flag/TFEB. Scale bar, 25 µm. b Lamp1 immunolabeling (blue) in ipsilateral SNpc of AAV-TFEB-injected rats (12 m post-AAV). *p < 0.05, compared to contralateral (non-injected) SNpc (Mann–Whitney rank sum test). Scale bar, 12.5 µm. c NM-laden neurons with p62-positive MB (left) and PB/LB-like inclusions (right) in ipsilateral SNpc from AAV-hTyr- and AAV-hTyr/TFEB-injected rats (2 m post-AAV). *p < 0.05, compared to AAV-hTyr-injected animals (left, Mann–Whitney rank sum test; right, two-way ANOVA, Student–Newman–Keuls post-hoc test). d Intracellular NM density in ipsilateral SNpc TH-positive neurons from rats co-injected with AAV-TFEB/AAV-hTyr (12 m post-AAV). White arrowhead, Flag/TFEB-positive nucleus; black arrowhead, Flag/TFEB-negative nucleus. Scale bar, 12.5 µm. *p < 0.05, compared to Flag/TFEB-negative neurons (Mann–Whitney rank sum test). e SNpc TH-positive neurons at 12 m post-AAV/vehicle injections. f Striatal TH-positive fibers at 12 m post-AAV/vehicle injections. In e, f, *p < 0.05, compared to respective contralateral side; #p < 0.05, compared to ipsilateral vehicle- and AAV-TFEB-injected animals; §p < 0.05, compared to ipsilateral AAV-hTyr-injected animals (two-way ANOVA; Student–Newman–Keuls post-hoc test). g TH downregulation within NM-laden neurons (12 m post-AAV). *p < 0.05, compared to AAV-hTyr-injected animals (two-tailed t-test). h Total SNpc DA neurons at 12 m post-AAV/vehicle injections. *p < 0.05, compared to respective contralateral side; #p < 0.05, compared to ipsilateral vehicle- and AAV-TFEB-injected animals (two-way ANOVA; Student–Newman–Keuls post-hoc test). i Contralateral forepaw use in AAV-hTyr- and AAV-hTyr/TFEB-injected rats (12 m post-AAV). *p < 0.05, compared to AAV-hTyr (only)-injected animals (two-tailed t-test). Dashed line indicates average contralateral forepaw use in control-injected rats. In all panels, values are mean ± SEM. In b, n = 138 neurons from n = 4 non-injected rats and n = 203 neurons from n = 5 AAV-TFEB-injected rats. In c, n = 6 (hTyr), n = 8 (hTyr + TFEB) rats. In d, n = 132 nuclear flag- neurons and n = 173 nuclear flag + neurons from n = 4 AAV-hTyr + TFEB-injected rats. In e and h, n = 7 (vehicle), n = 4 (TFEB), n = 5 (hTyr), n = 4 (hTyr + TFEB) rats. In f, n = 7 (vehicle), n = 6 (TFEB), n = 4 (hTyr), n = 3 (hTyr + TFEB) rats. In g, n = 4 animals per group. In i, n = 12 (control), n = 3 (hTyr), n = 4 (hTyr + TFEB) rats. BF bright-field. Photomicrographs correspond to 5-µm-thick sections
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Nature communications
March 07 2019

Fig 2: General proteostasis failure in NM-producing cells. a Top, Induction of hTyr expression in differentiated neuroblastoma SH-SY5Y cells before (OFF) and after (ON) treatment with doxycycline. Bottom, quantification of intracellular NM optical density post-hTyr induction. *p < 0.05, compared to OFF and ON 1d (ANOVA on ranks; Dunn’s post-hoc test). Scale bar, 12.5 µm. b Electron micrographs of NM granules in hTyr-expressing cells (6d post-hTyr induction). Arrowhead, double-membrane; asterisk, lipid droplet. Scale bar, 0.5 µm. c Immunoblot levels of Lamp1 (left) and LC3 (right) post-hTyr induction. d Lysosomal proteolysis post-hTyr induction. e Ubiquitin-proteasome (UPS) activity post-hTyr induction. f Immunoblot levels of p62 post-hTyr induction. g Representative images (left) and quantification (right) of aSyn oligomers (red) in hTyr-expressing cells as detected by aSyn-proximity ligation assay. Hoechst, nuclei (blue). BF bright-field. Scale bar, 25 µm. h Mitochondrial oxygen consumption rate (OCR) post-hTyr induction. i ROS production post-hTyr induction. MFI mean fluorescence intensity. j Cellular metabolic activity post-hTyr induction. k Number of surviving cells post-hTyr induction. l Representative bright-field photomicrographs with superimposed nuclear Hoechst fluorescent staining (blue) of hTyr-expressing cells before (OFF) and after (ON, 1–8d) hTyr induction. For clarity purposes, cell contour in weakly or no melanized cells are highlighted in white. Scale bar, 12.5 µm. In all panels, values are mean ± SEM. In a, d, e and i, n = 3 independent experiments. In c, h, j and k, n = 4 independent experiments. In a (bottom), n = 499 (OFF), n = 155 (1d), n = 111 (3d), and n = 48 (6d) cells. In g, n = 153 (OFF), n = 201 (3d), and n = 135 (6d) cells. Immunoblot densitometry was normalized to ß-actin expression levels. BF bright-field, KDa kilodaltons. Unstained NM appears as brown. In c–f, h, j, *p < 0.05, compared to OFF (one-way ANOVA; Student–Newman–Keuls post-hoc test). In g, *p < 0.05, compared to OFF; #p < 0.05, compared to ON 3d (ANOVA on ranks; Dunn’s post-hoc test). In i, *p < 0.05, compared to OFF; #p < 0.05, compared to ON 3d (one-way ANOVA; Student–Newman–Keuls post-hoc test). In k, *p < 0.05, compared to OFF (ANOVA on ranks; Dunn’s post-hoc test)

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Anti-LAMP1 antibody

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GeneTex's Anti-LAMP1 antibody is a Rabbit Polyclonal antibody. The Anti-LAMP1 antibody was generated using lysosomal associated membrane protein 1, CD107a, LAMPA, LGP120 as the antigen. The Anti-LAMP1 antibody from GeneTex is a Rabbit Polyclonal antibody to LAMP1. The Anti-LAMP1 antibody Recognizes Human, Mouse. The Anti-LAMP1 antibody has been validated for the following Applications: WB

Nature communications

Nature communications

Anti-LAMP1 antibody from GeneTex

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GeneTex

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Anti-LAMP1 antibody from GeneTex

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