Fig 1: The effect of PBNP-PTT-administered thermal dose on immune checkpoint molecules, an NK cell-activating ligand, and a neuroblastoma-associated antigen is more pronounced in SH-SY5Y cells compared with LAN-1 cells. (A–D) SH-SY5Y and (E–H) LAN-1 cells were treated in vitro with varied thermal doses via PBNP-PTT. 24 h post-PBNP-PTT, cells were analyzed for expression of (A,E) PD-L1, (B,F) B7-H3, (C,G) PVR, and (D,H) GD2 using flow cytometry analysis. Inset values in the histograms denote the thermal dose. Each parameter was compared to the vehicle control by one-way ANOVA, where * p < 0.03, ** p < 0.002, *** p < 0.0002, **** p < 0.0001; n = 2/group.