Fig 1: Prediction and validation of interface regions for Nup93-Nup205 (A) Cross-linking based mass spectrometry defined proximity regions between Nup93-Nup205 (adapted from Kosinski et. al, 2016 [51]). (B) Top 10% regions predicted by CoRNeA. Edges in bold depict three most significant regions (N-terminal of Nup93 with C-terminal of Nup205) (details in Table S7). (C) GFP-fused deletion constructs for Nup93 for validating the predictions. (D) Immunoprecipitation results depicting N-terminal region (1-150) and R2 regions (96-150) of Nup93 specifically interact with endogenous Nup205. GFPNB: GST-anti-GFP-nanobody.