Fig 1: Incomplete non-canonical processing due to loss of DmANGEL results in aberrant mitochondrial translation.a De novo translation in isolated mitochondria from 4-dael control (wDah) and DmAngelKO (KO 1,2) larvae. Mitochondrial proteins are indicated. Porin was used as loading control. A representative experiment is shown with n = 2 biologically independent samples, of two independent experiments performed. b Western blots of ribosome gradient fractions from 4-dael control and DmAngelKO larvae, decorated with antibodies against the small (MRPS16) and large (MRPL12) mitochondrial ribosome subunits. The small (28S), large (39S), and monosome (55S) fractions are indicated. A representative experiment is shown of four independent experiments performed with biologically independent samples. c, d Abundance of mitoribosome footprints in mitochondrial transcripts from ribosome profiling experiments in control and DmAngelKO as indicated, normalised to total read count and expressed as proportion of total footprints (n = 2 biologically independent samples).
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