Fig 1: Improvement of obesity-associated pancreas steatosis and inflammation after sleeve gastrectomy. Hematoxylin-eosin-stained pancreas sections showing pancreas steatosis in (A) rats with diet-induced obesity and (B) after surgical and dietary interventions (magnification 100X). Impact of diet-induced obesity as well as weight loss achieved by sleeve gastrectomy on pancreatic triacylglycerol (TG) content (C, D) and transcript levels of lipogenic transcription factors and enzymes (F, G). (E) Correlation between pancreatic Gucy2c mRNA and pancreatic steatosis. Representative images showing the brown staining for pan-macrophage marker CD68 in sections of the pancreas of (H) rats with diet-induced obesity and (I) after surgical and dietary interventions (magnification 200X); arrows indicate CD68+ cells. Bar graphs show the effect of diet-induced obesity and weight loss achieved after sleeve gastrectomy on macrophage infiltration (J, L) and gene expression of proinflammatory factors (K, M) in the pancreas. *P< 0.05; **P<0.01; ***P<0.001 vs lean or sham-operated rats.
Fig 2: Effect of obesity and weigh loss achieved by sleeve gastrectomy on the expression of the guanylin system in rat pancreas. (A) Cellular localization of the receptor of guanylin peptides, GUCY2C, in the islets of Langerhans (left panel, magnification 200X) as well as in inter- and intralobular pancreatic ducts (right panel, magnification 400X) in rat pancreas. Bar graphs illustrate the impact of weight gain induced by HFD feeding (B, C) and weight loss achieved by sleeve gastrectomy (D, E) on pancreatic mRNA and protein of GUCA2A, GUCA2B and their receptor GUCY2C. The gene and protein expression in lean control rats fed a ND or in the sham-operated group was assumed to be 1. Representative blots are shown. Statistical differences were analyzed using Student’s t-test or one-way ANOVA followed by a Tukey’s post hoc test, where appropriate. *P<0.05 vs lean or sham-operated rats.
Supplier Page from MilliporeSigma for Anti-GUCY2C antibody produced in rabbit