Fig 1: Validation of mRNA levels of representative genes by RT-qPCR for (A) focal adhesion pathway and (B) arachidonic acid metabolism pathway. Graphs show gene expression normalized to GAPDH as an endogenous control. In parallel, another two endogenous controls, 18S rRNA and HPRT1, were used with similar outputs. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns (non-significant). (C) Representative immunochemical analysis of COL4A1, VAV3, PTGS2, and AGR2. Beta-actin served as loading control.
Fig 2: (A) ELISA determination of PGE2 released from A549 cells into culture media. (B) Representative immunochemical analysis of COX-2 and AGR2. Beta-actin served as a loading control and for normalization to determine fold changes calculated by densitometric analysis in cells exposed to prostaglandin E2 (PGE2), diclofenac (DIC), celecoxib (CXB), and acetylsalicylic acid (ASA) compared to untreated cells (CTR). Significant changes (p < 0.05) determined from three independent biological experiments are indicated by asterisk.
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