Fig 1: Changes in the ECM composition of NHDF after a three-day RPM-exposure: Immunofluorescence images of 1g-control cells, RPM-AD and RPM-MCS of laminin (A–C) and fibronectin (D–F). Transcriptional and translational laminin analysis: Quantitative gene expression levels of LAMA3 (G), intracellular laminin levels (H) and flow cytometric analysis of laminin-labeled cells (I) displaying the percentage of laminin-positive cells as well as alteration of the median fluorescence intensity (MFI). Transcriptional and translational fibronectin analysis: Quantitative gene expression levels of FN1 (J), intracellular fibronectin levels (K) and flow cytometric analysis of fibronectin-labeled cells (L). Transcriptional and translational aggrecan analysis: Quantitative gene expression level of ACAN (M), intracellular aggrecan levels (N) and flow cytometric analysis of chondroitin sulfate-labeled cells (O). Transcriptional and translational osteopontin analysis: Quantitative gene expression levels of SPP1 (P), intracellular osteopontin levels (Q) and flow cytometric analysis of osteopontin-labeled cells (R). Full-length blots of cropped Western blot images are presented in Supplementary Fig. S1. *p < 0.05 1g vs. RPM; #p < 0.05 AD vs. MCS. Scale bars: 50 µm.