Fig 1: Overall and Disease-free survival based on high and low DCN and HSP90B1 staining.A, OS curve for DE. B, DFS curve for DE. C, OS curve for HE. D, DFS curve for HE. DE: Decorin staining in malignant epithelial tissue. HE: HSP90B1 staining in malignant epithelial tissue. OS: Time from diagnosis to death from any cause. DFS: Time from diagnosis to any recurrence or death from any cause.
Fig 2: Scoring system used for DCN and HSP90B1 immunohistochemistry.A, Strong DCN positivity in stroma (3+) and negative in carcinoma (0) (magnification 200×). B, Strong DCN positivity in carcinoma (3+), weak stromal positivity (1+) (200×). C, Moderate HSP90B1 positivity in carcinoma (1+) (200×). D, Strong HSP90B1 positivity in carcinoma (3+) (200×). Decorin antibody (Sigma-Aldrich, St. Louis, MO) used at a dilution of 1:400. HSP90B1 antibody (Sigma-Aldrich, St. Louis, MO) used at a dilution of 1:4000.
Fig 3: Identification and Isolation of Pure Stromal Subtypes in the Normal Human Prostate(A) scRNA-seq data aggregated from three normal prostate specimens subclustered into the stromal lineage.(B) Heatmap of the top 100 differentially expressed genes in each stromal subcluster with highlighted DEGs, suggesting putative identities.(C) qPCR of FACS-isolated stromal subtypes from three organ donor prostate specimens demonstrates the enrichment of cell type-specific DEGs.(D) Gene set enrichment analysis (GSEA) of non-endothelial stromal populations compared to KEGG pathways.(E) Immunofluorescent labeling of smooth muscle (MYH11), fibroblasts (DCN), and basal epithelia (KRT5).*p = 0.05; Scale bar, 100 µm. qPCR data are represented as mean ± SEM. Statistical significance of qPCR data was calculated by t test.
Supplier Page from MilliporeSigma for Anti-DCN antibody produced in rabbit