Fig 2: a–c Confocal laser scanning microscopy for the detection of ABCG5, NPC1L1 and ABCB1. Staining was carried out using Protocol #6. Cell nuclei are shown in blue (DAPI) and ABCG5, NPC1L1 or ABCB1 in green (fluorescently labelled antibody). Representative images of ABCG5 (a), treated with the vehicle control (DMSO 0.1%, left image) or GW3965 (LXR agonist, 10 µM, middle image). Representative images of NPC1L1 (b), treated with the vehicle control (DMSO 0.1%, left image) or GW3965 (LXR agonist, 10 µM, middle image). Representative images of ABCB1 (c), treated with the vehicle control (DMSO 0.1%, left image) or bexarotene (RXR agonist, 5 µM, middle image). The image on the right (a–c) is the respective control only stained with DAPI and the secondary antibody, showing the unspecific binding of the secondary antibody. Shown are maximum projections of the top views