Fig 1: SARS-CoV-2 proteins interact with mitochondrial PTP complex(A) COS-7 cells were cotransfected with HA-tagged PPIF and FLAG-tagged SARS-CoV-2 protein plasmid constructs. Following immunoprecipitation with HA antibody, total cell lysates and immunoprecipitated materials were subjected to western blot analysis. Cell lysates were probed with anti-FLAG or anti-HA antibodies. Immunoprecipitated samples were probed with anti-FLAG (top right) and anti-HA antibodies (bottom right). n = 3.(B) Western blot analysis of cell lysates (left) or immunoprecipitates (right) from COS-7 cells coexpressing HA-tagged SPG7 and FLAG-tagged SARS-CoV-2 protein plasmid constructs. n = 3.(C) Western blot analysis of cell lysates (left) or immunoprecipitates (right) from COS-7 cells coexpressing Myc-tagged CCDC58 and FLAG-tagged SARS-CoV-2 protein plasmid constructs. n = 3.(D) Western blots of cell lysates or immunoprecipitated material from COS-7 cells transiently coexpressing PPIF, SPG7, or CCDC58 proteins. Cells were lysed, immunoprecipitated with Myc antibody, and immunoblotted for HA. n = 3.(E) SARS-CoV-2 proteins and Mitochondrial PTP Complex binding. COS-7 cells were transfected with FLAG-tagged SARS-CoV-2 protein plasmid constructs. Following immunoprecipitation with FLAG antibody, SPG7, ANT, and ATP synthase interactions were assessed by western blot using specific antibodies.(F) Scheme depicts protein-protein interaction of mitochondrial PTP complex with SARS-CoV-2 proteins.