Fig 2: Effect of testosterone (10, 100 µM, incubation for 24 h) on OATP1B1 expression with simultaneous inhibition of FXR, PXR and LXRa. Ts—testosterone, Cntr—control, β-TA—FXR inhibitor, KTZ (ketoconazole)—PXR inhibitor, TFCA—LXRa inhibitor.

Fig 3: The role of orphan nuclear receptors in the inhibition of OATP1B1 under the action of 10 and 100 µM progesterone. (a) Results of Western blotting of OATP1B1 under the action of 10 µM progesterone + inhibitors of orphan receptors; (b) densitometric analysis of Western blotting of OATP1B1 under the action of 10 µM progesterone + inhibitors of orphan receptors; (c) results of Western blotting of OATP1B1 under the action of 100 µM progesterone + inhibitors of orphan receptors; (d) densitometric analysis of Western blotting of OATP1B1 under the action of 100 µM progesterone + inhibitors of orphan receptors. * p < 0.05, ** p < 0.01, *** p < 0.001, ANOVA, post hoc Dunnet’s test. Pg—progesterone, Cntr—control, β-TA—FXR inhibitor, KTZ (ketoconazole)—PXR inhibitor, TFCA—LXRa inhibitor.

Fig 4: The role of orphan nuclear receptors in the induction of OATP1B1 under the action of 10 and 100 µM testosterone. (a) Results of Western blotting of OATP1B1 under the action of testosterone 10 µM + inhibitors of orphan receptors; (b) densitometric analysis of Western blotting of OATP1B1 under the action of 10 µM testosterone + inhibitors of orphan receptors; (c) Results of Western blotting of OATP1B1 under the action of 100 µM testosterone + inhibitors of orphan receptors; (d) densitometric analysis of Western blotting of OATP1B1 under the action of 100 µM testosterone + inhibitors of orphan receptors. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001—statistically significant differences with the control, ANOVA, post hoc Dunnet’s test. Ts—testosterone, Cntr—control, β-TA—FXR inhibitor, CINPA1—CAR inhibitor, KTZ (ketoconazole)—PXR inhibitor, TFCA—LXRa inhibitor.