Fig 2: Assessing spatial organization of previously studied CEA cell types(A) Workflow for assessing the qualitative and quantitative spatial organization of cell types across CEA subdivisions, exemplified via Calcrl-expressing cluster. Left: Spatial subdivisions of the anterior CEA (top) and cells overlaid on top of CEA subdivisions (bottom). Middle: Cell-density map of Calcrl-expressing cell cluster for this individual section. Right: Enrichment analysis, across anterior sections from all animals, for Calcrl expression by subdivision.(B–D) As in (A), but for cells associated with all previously known cluster markers (i.e., Calcrl, Prkcd, Crh, Sst, Cxcl14). Scale bar: 500 µm.

Fig 3: Interrelationships of CEA cell-type identities and projection targets(A) Expression of Isl1 (green), Nr2f2 (red), and Prkcd (blue) marker genes in a representative section, as well as a three-color overlay illustrating relatively discrete spatial domains.(B) Overview of expression of genes in (A), combined with circuit-mapping projections from the substantia nigra (white, with results depicting a single optical section from the intermediate CEA). Inset illustrates the expansion of an example projection cell, denoted by arrow in overlay. Scale bars: 100 µm for overview, 10 µm for expansion.(C) Aggregate statistics for expression of Isl1, Nr2f2, and Prkcd, conditioned by projecting cell vs. non-projecting cells, for CEAm projectors to the substantia nigra, parabrachial nucleus, and periaqueductal gray. Data reflect the analysis of anterior and intermediate sections from n = 2 animals per projection.(D) As in (C), but for relatively diffuse projections to the lateral hypothalamus.