Fig 1: Altered expression and localization of proteins relevant to lacrimal gland fusion and secretion. (A) Rab3d immunofluorescence was abundant in the apical lumen side of the acinar cells in the sham LGs (left panels), but uniformly distributed in the STDOB (middle panels) and more basally localized in the Prickle1 mutant acini. The bottom panels are magnified images from the top panels. Apical and basal positions of the acinus lumen are illustrated in the right corner of the first panel. (B) Western blotting demonstrated reduced Rab3d expression in the STDOB and Prickle1 mutant LGs compared with the sham. (C) Vamp8 immunofluorescence showed a similar altered pattern as the Rab3d in the STDOB and Prickle1 mice. (D) Western blotting showed increased LG expression of Vamp8 in the STDOB and Prickle1 mice. (E) Expression of Snap23 in LG acini was also basally shifted and showed increased levels in STDOB and Prickle1 mice compared with the sham (F). The white dotted lines indicate acini boundaries. For Western blotting and quantification, n = 4 LGs/mice.