Fig 1: S100A14 was the direct target for miR-335-5p.Notes: (A) Binding module between S100A14 and miR-335-3p. (B) S100A14 expression in NSCLC cells with synthetic miRNAs transfection. (C) S100A14 protein expression in NSCLC cells with synthetic miRNAs transfection. (D) Relative luciferase activity in NSCLC cells with synthetic miRNAs and luciferase activity vectors transfection. (E) RIP assay revealed CASC9, miR-335-3p, and S100A14 were co-enriched in anti-Ago2 pellets. RT-qPCR results showed S100A14 was increased in NSCLC (F) tissues and (G) cell lines. Western blot to analyze S100A14 protein expression in NSCLC (H) tissues and (I) cell lines. (J) IHC assay to detect S100A14 expression in NSCLC tissues and normal tissues. (K) Correlation of S100A14 and miR-335-3p in NSCLC tissues. ***P < 0.001; **P < 0.01.Abbreviations: RT-qPCR, quantitative real-time PCR; NSCLC, non-small cell lung cancer; S100A14, S100 calcium binding protein A14; miR-335-3p, microRNA-335-5p; miR-NC, negative control microRNA; wt, wild-type; mt, mutant.
Fig 2: CASC9 promotes NSCLC progression through S100A14.Notes: (A) RT-qPCR to detect S100A14 expression after si-CASC9+pcDNA3.1, si-CASC9+pS100A14, siR-NC+pcDNA3.1, or siR-NC+pS100A14 transfection. (B) Cell counting kit-8 assay showed that S100A14 overexpression accelerates NSCLC cell proliferation. (C) Wound-healing assay showed S100A14 overexpression increased cell migration ability. (D) Transwell assay showed pS100A14 transfection enhanced invasion ability. ***P < 0.001; **P < 0.01; *P < 0.05.Abbreviations: RT-qPCR, quantitative real-time PCR; NSCLC, non-small cell lung cancer; CASC9, cancer susceptibility candidate-9; S100A14, S100 calcium-binding protein A14; si-CASC9, small interfering RNA targeting cancer susceptibility candidate-9; siR-NC, negative control small interfering RNA.
Supplier Page from Abcam for Anti-S100A14 antibody