Fig 2: High TCIRG1 expression is associated with unfavorable overall survival for GBM. (A and B) Kaplan-Meier curve analyses showed that patients with GBM with high TCIRG1 expression were more likely to have poor OS in the TCGA-GBM and GSE16011 datasets. TCIRG1, T cell immune regulator 1; TCGA, The Cancer Genome Atlas, GBM, glioblastoma multiforme.

Fig 3: Correlation between TCIRG1 expression and immune cell infiltration and immune scores in GBM. (A) TCIRG1 expression was negatively significantly correlated with tumor purity and CD8+ T cell infiltration but positively significantly correlated with CD4+ T cell and dendritic cell infiltration levels. No statistical significance was found in B cells, macrophages or neutrophils. Increased TCIRG1 expression predicted (B) high stromal scores (B), (C) high immune scores and (D) high ESTIMATE scores in GBM samples. ****P<0.0001. TCIRG1, T cell immune regulator 1; cor, correlation.

Fig 4: TCIRG1 expression in different types of cancer. (A) Expression levels of TCIRG1 in cancer and normal tissues in the Oncomine database. (B) TCIRG1 expression data from TCGA were compared using the TIMER database. Increased TCIRG1 expression was found in GBM according to data from (C) CGGA, (D) GSE16011 and (E) TCGA-GBM. (F and G) Protein levels of TCIRG1 were validated using western blot experiments, and increased expression was found in the glioma group. ****P<0.0001 vs. WHO III or LGG, **P<0.01 and *P<0.05 vs. respective control. TCIRG1, T cell immune regulator 1; ns, no statistical significance; CGGA, Chinese Glioma Genome Atlas; TCGA, The Cancer Genome Atlas, GBM, glioblastoma; WHO, World Health Organization; TPM, transcripts per million.

Fig 5: GO analysis of genes that showed positive and negative correlations with TCIRG1 in The Cancer Genome Atlas-Glioblastoma cohort. Terms for (A) GO-BP, (B) GO-CC and (C) GO-MF of genes that showed positive correlations with TCIRG1. Terms for (D) GO-BP, (E) GO-CC and (F) GO-MF of genes that showed negative correlations with TCIRG1. Red nodes represent gene counts, and black bars represent -Log10 (P-value). GO, Gene Ontology; BP, biological process; CC, cellular component; MF, molecular function.