Anti-γ-Actin antibody, Mouse monoclonal A8481 from MilliporeSigma

Product Specs
ItemAnti-γ-Actin antibody, Mouse monoclonal
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA8481
Quantity200µL
Applicationsindirect ELISA, western blot, immunohistochemistry
Conjugate/TagUnconjugated
Reactivitybovine, human, canine, mouse, hamster, chicken
TargetACTG1; chicken RCJMB04_1h13; bovine ACTG1; canine LOC475123; mouse Actg1
Clone2-2.1.14.17
Hostmouse
ClonalityMonoclonal
Concentration~2 mg/mL
NCBI Full Gene Nameactin, gamma, cytoplasmic 1, Actin, gamma 1, actin gamma 1
NCBI Gene AliasesAL023024, DFNA26, Actl, DFNA20, ACT, HEL-176, Actg, E51, ACTG

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Website: www.SigmaAldrich.com/

(1) Targeting local lymphatics to ameliorate heterotopic ossification via FGFR3-BMPR1a pathwayNature communicationsJuly 19, 2021Zhang D, Huang J, Sun X, Chen H, Huang S, Yang J, Du X, Tan Q, Luo F, Zhang R, et al.ab38560, Abcam), pSmad1/5 (1:500; 700047, Thermo), Smad1/5 (1:1000; ab75273, Abcam), β-actin (1:5000; A8481, Sigma-Aldrich). Chemiluminescence was performed with SuperSignal West Dura Extended Duration Substrate

(2) Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagyRedox biologySeptember 1, 2020Wu H, Wei H, Zhang D, Sehgal SA, Zhang D, Wang X, Qin Y, Liu L, Chen Q.(ProteinTech, 19998-1-AP), α-Flag (Sigma-Aldrich, F1804), α-Myc (Sigma-Aldrich, M4439), α-Actin (Sigma-Aldrich, A8481). The rabbit α-FUNDC1 and rabbit α-F1-P13 phosphorylation polyclonal antibodies were generated by immunizing

(3) Formation of Aberrant Myotubes by Myoblasts Lacking Myosin VI Is Associated with Alterations in the Cytoskeleton Organization, Myoblast Adhesion and FusionCellsJuly 11, 2020Lehka L, Topolewska M, Wojton D, Karatsai O, Alvarez-Suarez P, Pomorski P, Rędowicz MJ.mouse monoclonal to desmin (BD Biosciences 550626, 1:1000); mouse monoclonal to γ-actin (Sigma-Aldrich A8481, 1:1000); mouse monoclonal to β-actin (Sigma-Aldrich A5441, 1:1000); rabbit monoclonal to dysferlin

(4) EGCG protects vascular endothelial cells from oxidative stress-induced damage by targeting the autophagy-dependent PI3K-AKT-mTOR pathwayAnnals of Translational MedicineApril 21, 2020Jiao Meng, Yuhua Chen, Junzhe Wang, Junling Qiu, Cuicui Chang, Fangfang Bi, Xiaopeng Wu, Wei LiuCell Signaling Technology (CST, Danvers, MA, USA). Antibodies against 3-methyladenine (3-MA) (M9281) and β-actin (A8481) were from Sigma. Briefly, HUVECs were lysed, then quantified proteins using the BCA assay (23225, Thermo Scientific

(5) GABARAPL2 Is Critical for Growth Restriction of Toxoplasma gondii in HeLa Cells Treated with Gamma InterferonInfection and immunityApril 20, 2020Zhang Z, Gu H, Li Q, Zheng J, Cao S, Weng C, Jia H.Proteintech. Polyethylenimine (PEI) and Abs against ATG5 (A0856), green ﬂuorescent protein (GFP) (SAB4301138), and ␤-actin (A8481) were purchased from Sigma-Aldrich. Abs against GABARAPL2 (D1W97) and HA (C29F4) were purchased from

(6) STING directly activates autophagy to tune the innate immune responseCell death and differentiationSeptember 1, 2019Liu D, Wu H, Wang C, Li Y, Tian H, Siraj S, Sehgal SA, Wang X, Wang J, Shang Y, et al.The following antibodies were purchased from Sigma: anti-LC3 (L8918), anti-Flag (F1804), anti-Actin (A8481), and anti-HA (H9658). The rabbit anti-ATG9A (ab108338) and anti-p-TBK1 (Ser172, ab109272) were bought

(7) EGFP insertional mutagenesis reveals multiple FXR2P fibrillar states with differing ribosome association in neuronsBiology openAugust 21, 2019Stackpole EE, Akins MR, Ivshina M, Murthy AC, Fawzi NL, Fallon JR.primaries at 4°C (anti-FXR2P clone 55; 1:1000; BD Biosciences; or anti-γ-actin; 1:40,000, Sigma-Aldrich #A8481). Blots were washed four times for 5 min each in wash buffer after primary incubation. Horseradish peroxidase

(8) The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritisCell death & diseaseJuly 8, 2019Ni Z, Kuang L, Chen H, Xie Y, Zhang B, Ouyang J, Wu J, Zhou S, Chen L, Su N, et al.IL-1β-antibody (#12242, Cell signaling), Cleaved-IL-1β-antibody (#83186, Cell signaling), ACTB/β-actin-antibody (A8481, Sigma-Aldrich), LC3B-antibody (#3868,Cell signaling), ATG4B-antibody (15131–1-AP, Proteintech), ATG5-antibody

(9) Variable cytoplasmic actin expression impacts the sensitivity of different dystrophin‐deficientmdxskeletal muscles to eccentric contractionThe FEBS JournalJuly 1, 2019Angus Lindsay, William M. Southern, Preston M. McCourt, Alexie A. Larson, James S. Hodges, Dawn A. Lowe, James M. Ervastiantibodies used were anti-utrophin at 1:50 (DSHB, MANCHO3), anti-cytoplasmic γ-actin at 1:1000 (Sigma, A8481), anti-cytoplasmic β-actin at 1:1000 (Sigma, A1978), anti-SERCA1 at 1:1000 (Abcam, ab2819) anti-GAPDH

(10) NRF3 suppresses breast cancer cell metastasis and cell proliferation and is a favorable predictor of survival in breast cancerOncoTargets and TherapyApril 18, 2019Jianguo Sun, Zhibao Zheng, Qi Chen, Yin Pan, Hongsheng Lu, Hui Zhang, Yingzhi Yu, Yuechu Daianti-p- NF-κB p65 (S536) (CST, 3033) and anti-ID3 (CST, 9837) diluted 1:1000, anti-β-actin (Sigma, A8481) diluted 1:5000. Protein bands were detected with an ECL chemiluminescence reaction kit (Thermo Scientific). NRF3

(11) Fibroblast Growth Factor-1 Improves Insulin Resistance via Repression of JNK-Mediated InflammationFrontiers in pharmacologyJanuary 1, 2019Fan L, Ding L, Lan J, Niu J, He Y, Song L.(C-17) were purchased from Santa Cruz Biotechnology. Antibodies to Flag (Cat. F2555) and Actin (Cat. A8481), anti-Flag beads (Cat. M8823), and lipopolysaccharide (LPS, Cat. L2630) were purchased from Sigma-Aldrich.

(12) miR‑27a promotes human breast cancer cell migration by inducing EMT in a FBXW7‑dependent mannerMolecular Medicine ReportsOctober 24, 2018Guobin Jiang, Weiwu Shi, Hongyan Fang, Xiaohua Zhangpurchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). The antibody against β-actin (cat. no. A8481; 1:5,000) was obtained from Sigma-Aldrich (Merck KGaA) and used as a loading control in clinical specimen

(13) Knockdown of long non-coding RNA LCPAT1 inhibits autophagy in lung cancerCancer Biology & MedicineSeptember 11, 2018Xiao Xiao, Xiaofei Xiaofei, Hongyan Hongyan, Nannan Nannan, Sumeng Sumeng, Xiaohong Xiaohong, Wang Wang, Herbert Herbert, Xiaobei Xiaobei, Biyun BiyunAbcam), anti-ATG5 (#ab108327, 1:500; Abcam), anti-BECLIN1 (#ab55877, 1:500; Abcam), and anti-β-ACTIN (#A8481, 1:4000; Sigma-Aldrich). After washing, the membranes were incubated with a secondary antibody and imaged

(14) ATF3 suppresses ESCC via downregulation of ID1Oncology LettersSeptember 1, 2016Jian Li, Zishan Yang, Zhiuguo Chen, Yonghua Bao, Huijuan Zhang, Xinhui Fang, Wancai YangUSA), glyceraldehyde 3‑phosphate dehydrogenase (cat no. G8795, dilution 1:500) and β‑actin (cat no. A8481, dilution 1:1000; Sigma‑Aldrich). Secondary antibodies (cat no. SC-2004, goat anti-rabbit IgG-HRP, dilution

(15) Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrierNature communicationsJune 23, 2016Campos Y, Qiu X, Gomero E, Wakefield R, Horner L, Brutkowski W, Han YG, Solecki D, Frase S, Bongiovanni A, et al.anti-β actin (600-401-886S, Rockland, 1:500 WB), anti-γ tubulin (T5192, Sigma, 1:750 IF), anti-γ actin (A8481, Sigma, 1:500 IF), E-cadherin (3195, Cell Signaling, 1:500 WB), anti-pan actin (4968, Cell Signaling,

(16) Inhibition of Histone Deacetylase 3 (HDAC3) Mediates Ischemic Preconditioning and Protects Cortical Neurons against Ischemia in RatsFrontiers in molecular neuroscienceJanuary 1, 2016Yang X, Wu Q, Zhang L, Feng L.(ab47237, Abcam), anti-lamin A/C (2032, Cell Signaling Technology, Beverly, MA, USA), anti-β-actin (A8481, Sigma–Aldrich), anti-Bcl-xL (2764, Cell Signaling Technology), anti-Prdx2 (10545-2-AP, Proteintech Group

(17) Mapping synapses by conjugate light-electron array tomographyThe Journal of neuroscience : the official journal of the Society for NeuroscienceApril 8, 2015Collman F, Buchanan J, Phend KD, Micheva KD, Weinberg RJ, Smith SJ.Cell Signaling Technology 5843 Biosciences Pharmingen 612632 Abcam ab18251 Sigma-Aldrich T6793 Abcam 41489 Sigma-Aldrich A8481 BD Biosciences 610517 Abcam ab28172 GeneTex GTX13970 AVES MBP 1:200 1:15 1:5000 1:200 1:200 1:10000 1:200 1:100 1:100 1:100 1:200 1:100 1:25 1:100 1:100 1:100 Table

(18) Synaptic molecular imaging in spared and deprived columns of mouse barrel cortex with array tomographyScientific DataDecember 1, 2014Nicholas C Weiler, Forrest Collman, Joshua T Vogelstein, Randal Burns, Stephen J SmithSignaling,C26D12,"2,173",1:100 3. GABAARa1,Mouse,mono,NeuroMABs,N95/35,75–136,1:100 4. gActin,Mouse,mono,Sigma,2-2.1.14.17,A8481,1:100 5. GAD2,Rabbit,mono,Cell Signaling,D5G2,"3,988",1:200 6. GAD6567,Rabbit,poly,AbCAM,N/A,AB11070,"1:1,000" 7.

(19) Simultaneous detection of multiple targets for ultrastructural immunocytochemistryHistochemistry and Cell BiologyMarch 1, 2014V. V. Philimonenko, A. A. Philimonenko, I. Šloufová, M. Hrubý, F. Novotný, Z. Halbhuber, M. Krivjanská, J. Nebesářová, M. Šlouf, P. Hozák(nucleophosmin) rabbit polyclonal antibody (ab15440 from Abcam), anti-γ-actin mouse monoclonal IgG (#A8481 from Sigma), and guinea pig anti-SMC2 kindly provided by W. Earnshaw (Saitoh et al. 1994). The following

(20) Mena/VASP and αII-Spectrin complexes regulate cytoplasmic actin networks in cardiomyocytes and protect from conduction abnormalities and dilated cardiomyopathyCell communication and signaling : CCSAugust 12, 2013Benz PM, Merkel CJ, Offner K, Abeßer M, Ullrich M, Fischer T, Bayer B, Wagner H, Gambaryan S, Ursitti JA, et al.(T8328, Sigma), anti-β-cytoplasmic actin (13E5, Cell Signalling; A1978, Sigma), anti-γ-cytoplasmic actin (A8481, Sigma), anti-CapZ (AB6017, Millipore), anti-Cx43 (sc-6560, Santa Cruz; 710700, Invitrogen), anti-α-Actinin

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Anti-γ-Actin antibody, Mouse monoclonal from MilliporeSigma

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Anti-γ-Actin antibody, Mouse monoclonal from MilliporeSigma

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