Fig 1: M-ATOs Recapitulate T Cell Differentiation from Different Hematopoietic Subsets(A–C) Different subsets in the hematopoietic hierarchy from HSCs to T cell progenitors were isolated from the bone marrow and the thymus of C57BL/6 WT mice and seeded into M-ATOs. From the bone marrow: HSC (hematopoietic stem cell) (Lin-Sca1+cKit+CD48 CD150+IL7R-); MPP (multi-potent progenitor) (Lin-Sca1+c-Kit+CD48-CD150-IL7R-); LMPP (lymphoid-primed multi-potent progenitor) (Lin-Sca1+c-Kit+IL7R+Flk2+); and CLP (common lymphoid progenitor) (Lin-Sca1Loc-KitLo IL7R+Flk2+). From the thymus: ETP (Lin-CD4-CD8-c-Kithi CD44hiCD25-); DN2 (Lin-CD4-CD8-c-Kithi CD44hi CD25+); and DN3 (Lin-CD4-CD8-CD44-CD25+). Representative phenotypes of M-ATO-derived cells are shown at weeks 1 (A), 2 (B), and 6 (C). Data are representative of three biological replicates.(D) Frequencies of T cell populations shown as percentage of total CD45+ TCR?d- Lin- cells initiated from the different hematopoietic subsets in week 1, week 2, and week 6 M-ATOs. Error bars denote ± SD (n = 3 independent experiments).