Fig 1: JASMONATE RESISTANT1 (JAR1) is induced in the abscission zone (AZ) cells of yellow lupine flowers in response to soil drought. AZ from flowers collected from 48 day-old plants growing under optimal soil moisture (70% water holding capacity, WHC) was a control, while the “drought” variant was AZ excised from 48 day-old plants subjected to drought (25% WHC), as written precisely in the Material and Methods section. Western blot analysis was performed with a JAR1 antibody and revealed the presence of a reactive band of ~64 kDa (A). The membrane was scanned, densitometry analysis was performed (100% was set for the control), and presented in (B). Protein samples separated by SDS-PAGE and stained with Coomassie Brilliant Blue (C). Protein marker (M) is displayed on the left and sizes in kDa are provided. Immunolocalization of JAR1 in flower AZ cells during soil water deficit (E) and under control conditions (D). Green fluorescence indicates JAR1 localization (marked by red arrows). Blue labeling corresponds to DAPI-stained nuclei. AZ region is restricted by white dotted lines. Abbreviation: VB—vascular bundle. Bars = 40 µM. A significant difference is ^^ p < 0.01.