Fig 2: (a) Mechanism of antiaging/atresia of Met via the calcium ion (Ca2+) transport signaling pathway in the H2O2-induced ASWFs. A: the effect of Met on the expression of GRP75, Cyt c, and MFN2 with IF and IHC analyses. Scale bar: 50 µm. B: Western blot and grey analysis of IP3R, caspase3, GRP75, CAMKII, MFN2, and Cyt c expression in ASWFs. C: effect of Met on expression of Ca2+ transport-related genes. (b) Mechanism of antiaging/atresia of Met via the insulin signaling pathway. A: the effect of Met on expression of PI3K (red), BAD (green), and DAPI (blue) with IF. Scale bar: 50 µm. B: Western blot and grey analysis of p-AKT/AKT and p-GSK-3ß/GSK-3ß expression in ASWFs. C: effect of Met on expression of PI3K, BCL2, and GLUT4 mRNAs in ASWFs. (c) Mechanism of antiaging/atresia of Met via the lipid metabolism signaling pathway. A: Western blot and grey analysis of PPAR-?, occludin, and VLDLR expression in ASWFs. B: qRT-PCR analysis of PPAR-?, occludin, and VLDLR expression in ASWFs. Values represent the means ± SEM of three replicates in each group. Different lowercase letters indicate significant difference (P < 0.05).

Fig 3: Analysis of the interaction of PI3K-AKT pathway by Western blot. (A)Quantitative protein expression levels of p-PI3K, PI3K, P65, p-AKT, AKT, Bcl-2 and Bax.(B) The p-PI3K/PI3K ratio in cardiac tissue injury. (C) The ratio of p-AKT/AKT in cardiac tissue injury. (D) Protein expression levels of Bcl-2. (E) Protein expression levels of Bax. (F) The protein expression level of P65. (G) Protein expression level of p-PI3K, PI3K. (H) Protein expression level of p-AKT, AKT.

Fig 4: The mechanism of Schisandra chinensis combined with coenzyme Q10 inhibiting heart failure through PI3K/AKT signal pathway.

Fig 5: The PI3K and SGK1 inhibitor-treated BMDM CM could rescue CF phenotypes. a, b Representative western blot images of collagen I, collagen III, Smad2/3, and phospho Smad2/3 and c relative expression in different groups. D: Representative fluorescence images of CFs and g the relative fluorescence in different groups. *p < 0.05, **p < 0.01