Fig 1: DKK1 expression is related to AFP in AFPGC. (A) Bubble chart showing the top 15 genes related to AFP expression via correlation analysis. (B) Scatter plots showing the correlation between AFP expression and three selected genes using TCGA gastric cancer dataset. (C) Violin plot shows DKK1 expression level among malignant epithelium clusters. (D) Consensus clustering divided 341 cases (from TCGA‐STAD cohort) into two groups, namely the AFPGC group (45 cases) and common gastric cancer (GC) group (296 cases). (E) Bubble chart showing the enriched pathway of AFPGC upregulated genes. (F) Bar diagram showing the expression level of several marker genes and hepatocyte score based on GSVA. (G) Correlation between AFP and DKK1 expression among 45 AFPGC patients in TCGA cohort. (H) HE stains and immunohistochemistry on another two AFPGC tissues. All pictures were captured at a magnification of 100×. (I) Western blot showing AFP, ALB, GPC3, and several EMT marker expression in FU97 cells with or without DKK1 stimulation. RT‐qPCR was performed to detect AFP transcription level. FU97 was treated with DKK1 (0.1 μg/mL) and incubated for 48 h before extracting protein and total RNA.