Fig 1: Construction of single-cell landscapes of the forestomach tissues. (A, B, D) t-SNE maps of the rumen (A), reticulum (B), and omasum (D) single-cell data. Cells are colored by cell types. (C, E) Heatmap showing the representative highly expressed marker genes for epithelial cell subtypes in the reticulum (C) and omasum (E). Color scale: red, high expression; blue, low expression. (F) Coimmunostaining of KRT6A with GJA1 in reticulum and omasum tissues. Scale bars, 20 µm. (G) Coimmunostaining of KRT14 and MKI67 in rumen tissues. Scale bars, 20 µm. BC: basal cell; BVEC: blood vascular endothelial cell; cg-like SC: channel-gap-like spinous cell; GC: granule cell; LEC: lymphatic endothelial cell; MC: mitotic cell; SC: spinous cell. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)