Fig 1: AKT pathway signaling is suppressed by sh-IGFL2-AS1. (A) Schematic depicting the IGFL2-AS1 pathway. (B) Relative transcription of ARPP19, measured using qRT PCR. The graph depicts the levels of ARPP19 in the indicated cell lines. Data are normalized to those of the sh-NC-HT29 cell line; error bars indicate SDs. *** p < 0.001. (C) Protein expression of ARPP19, measured by western blotting. The expression of β-actin was used as a loading control. (D) Western blot analyses of various proteins downstream of AKT. Cells incubated as indicated after irradiation (3 Gy) were lysed and analyzed by western blotting; β-actin was used as a loading control. (E–H) Densitometric measurements of protein expressions, corresponding to (D). The results are presented as the ratio of phosphor-AKT/AKT (E), cyclin D1/β-actin (F), c-Myc/β-actin (G), and E-cadherin/β-actin (H).