Fig 2: Analysis of PAK complex formation using native electrophoresis. HEK293T cell lysate from cells transfected with plasmids encoding PAK1-full-eGFP, PAK1?15-eGFP or PAK2-eGFP were resolved on electrophoretic gels under native (left) or denaturating conditions (right). MW markers (kDa) are shown on the left for both gels. For the denaturating conditions, one representative sample from several replicates, which were run in the same gel, is shown for each isoform. The full length gel is shown in Supplementary Fig. S16. Lanes: 1 – PAK1-full, 2 – PAK1?15, 3 – PAK2.

Fig 3: Knockdown of hsa_circ_0004396 inhibited tumor growth in NSCLC in vivo. (A and B) The tumor volume and weight were measured. (C and D) hsa_circ_0004396 and miR-615-5p were detected by qRT-PCR. (E and F) The mRNA and protein levels of PAK1 were detected by qRT-PCR and Western blot. (G and H) The expression levels of Ki67 and PAK1 were examined by Immunohistochemical staining. *p < 0.05

Fig 4: Hsa_circ_0004396 boosted NSCLC cell malignant behavior and radiosensitivity, by regulating the miR-615-5p/PAK1 axis

Fig 5: circLMTK2 knockdown inhibits the growth of PC tumor in vivo. (a-b) The volume of tumors was recorded. (c-d) The mRNA expression levels of circLMTK2 and miR-485-5p were determined in tumor tissues. (f) The protein expression of PAK1 were determined in tumor tissues. **P < 0.01; ***P < 0.001 vs. NC group; ###P < 0.001 vs. GEM-resistant group.