Fig 1: NBTI mitigated cell apoptosis at 24 h after subarachnoid hemorrhage (SAH). (a) representative microphotographs of TUNEL staining in the injured cerebral cortex at 24 h after SAH. (b) Quantitative analysis of TUNEL-positive cell. (c) Representative western blotting analysis of equilibrative nucleoside transporter 1 (ENT1), Bcl2 and Bax levels in sham, SAH+vehicle and SAH+NBTI. (d) Quantitative analyses of ENT1. (e) Quantitative analyses of Bcl2. (f) Quantitative analyses of Bax. n = 4 per group. The bars represent the mean ± SD. *P < 0.05 versus sham. # P < 0.05 versus SAH+vehicle. NBTI, nitrobenzylthioinosine; TUNEL, TdT-mediated dUTP nick-end labeling.
Fig 2: ENT1 protein expression levels in the hippocampus of epileptic and con rats. (A) ENT1 protein expression levels were detected by western blot analysis. The relative optical density values of ENT1 protein bands were normalized to those of β-actin. (B) Bar graphs demonstrating ENT1 expression levels in the hippocampus of epileptic and con rats. ENT1 protein expression was upregulated in the SE group 3 days after seizure induction. SB203580 inhibited ENT1 protein expression levels compared with that in SE rats 3 days after seizure induction, whereas DMSO did not have this effect. n=5 in each group. *P<0.05 vs. SE; #P<0.05 vs. con. ENT1, type 1 equilibrative nucleoside transporter; SE, status epilepticus; con, control.
Fig 3: SB203580 inhibits the activation of p-p38 MAPK, regulates A1R and ENT1, and decreases the number of seizures and brain tissue damage. Dashed arrows represent suppression. p-, phosphorylated; A1R, adenosine A1 receptor; ENT1, type 1 equilibrative nucleoside transporter.
Fig 4: Changes in the expression levels of equilibrative nucleoside transporter 1 (ENT1), NLRP3 and Bcl2 within 72 h after subarachnoid hemorrhage (SAH). (a) Typical image of SAH. (b) Representative western blotting analysis of ENT1, NLRP3 and Bcl2 at 3, 12, 24 and 72 h after SAH. (c) Quantitative analyses of ENT1. (d) Quantitative analyses of NLRP3. (e), Quantitative analyses of Bcl2. n = 4 per group. The bars represent the mean ± SD. *P < 0.05 versus sham group.
Fig 5: ENT1 protein expression levels in the cortex of epileptic and con rats. (A) ENT1 protein was detected by western blot analysis. The relative optical density of ENT1 protein bands was normalized to that of β-actin. (B) Respective bar graphs showing ENT1 protein expression levels in the cortex of epileptic and con rats. ENT1 protein expression levels were upregulated in the SE group 3 days after seizure induction. SB203580 inhibited ENT1 protein expression levels compared with SE rats 3 days after seizure induction, whereas DMSO did not have this effect. n=5 in each group. *P<0.05 vs. SE; #P<0.05 vs. con. ENT1, type 1 equilibrative nucleoside transporter; con, control; SE, status epilepticus.
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