Fig 2: The expression levels and prognostic value of IGF2BP3. A The comparison of IGF2BP3 expression levels between the tumor and normal tissues. B IGF2BP3 expression levels in different tumor cell lines from the Cancer Cell Line Encyclopedia (CCLE) database. C The correlation between the programmed cell death ligand 1 (PD-L1) and IGF2BP3 mRNA expression levels from the CCLE database. D IGF2BP3 expression levels in the Oncomine database. P value threshold was set 0.05 and the fold-change threshold was set 2.0. The number in the colored cells represents the number of studies meeting the thresholds. The printed red (over-expression) or blue colors (under-expression) indicate a significant association. E The relation of IGF2BP3 with PD-L1 from the TIMER website. F The Kaplan–Meier curve shows the prognostic values of IGF2BP3 in patients with BCa obtained via Gene Expression Profiling Interactive Analysis (GEPIA), which was based on TCGA database. G, H The anatomical structure showed the expression levels of the IGF2BP3 in the normal organ tissues in males (G) and females (H). I Histogram visualizing the expression levels of IGF2BP3 in normal organ tissues. J Histogram visualizing the expression levels of IGF2BP3 in normal organ tissues between the females and males. *P < 0.05, ***P < 0.001

Fig 3: c-Myb promotes immune escape of EAC cells (FLO-1 cell line) by upregulating the expression of miR-145-5p. (A) c-Myb, SPOP, and PD-L1 expression in each group detected by Western blot analysis. (B) The expression of c-Myb, miR-145-5p, SPOP, and PD-L1 in each group detected using qPCR. (C) Tumor size of EAC tumor models in each group. (D) IHC diagrams of CD3 expression in EAC tissues in each group and statistical analysis of IHC scores. (E) IHC diagrams of Granzymes B expression in EAC tissues in each group and statistical analysis of IHC scores. (F) The expression of Fas and FasL in tumor tissues of each group detected by Western blot analysis. (G) The levels of cytokines secreted by T cells in each group detected using ELISA. (H) The proportion of cytokines secreted by Th1 and Th2 cells in each group detected using specific kits. (I) The proportion of CCR5 and CD4 double positive cells detected using flow cytometry. (J) The proportion of CCR3 and CD4 double positive cells assessed using flow cytometry. (K) The proportion of CCR8 and CD4 double-positive cells assessed using flow cytometry. *P < .05. Measurement data were expressed as mean ± standard deviation. Data in panels A-E were analyzed by unpaired t-test, in panels F-H were analyzed by one-way ANOVA, and in panels I-K were analyzed by two-way ANOVA and Tukey's post-hoc test

Fig 4: Graphical Abstract. c-Myb can up-regulate miR-145-5p expression and inhibit the SPOP-mediated ubiquitination and degradation of PD-L1 to strengthen of EAC cells through the SPOP/PD-L1 pathway

Fig 5: c-Myb induces immune escape of EAC cells (FLO-1 cell line) by promoting miR-145-5p transcription to inhibit the SPOP-mediated ubiquitination and degradation of PD-L1. (A) c-Myb, SPOP, and PD-L1 expression in each group assessed by Western blot analysis. (B) The expression of c-Myb, miR-145-5p, SPOP, and PD-L1 in each group detected using qPCR. (C) Proliferation of T cells cocultured with different groups of FLO-1 cells examined using CFSE dye and CD3 antibody. (D) Apoptosis of T cells cocultured with different groups of FLO-1 cells detected using flow cytometry combining with CD3 antibody. (E) The expression of E-cadherin, N-cadherin, MMP-9, and MMP-3 in FLO-1 cells in each group detected by Western blot analysis. (F) The invasion and migration of different groups of FLO-1 cells detected by Transwell assay. (G) The expression of Fas and FasL in FLO-1 cells of each group detected using Western blot analysis. (H) Apoptosis of FLO-1 cells in different groups assessed by flow cytometry. (I) The cytokine levels secreted by T cells in each group detected by ELISA. *P < .05. Measurement data were expressed as mean ± standard deviation. Data in panel C were analyzed by two-way ANOVA, and in other panels were analyzed by one-way ANOVA and Tukey's post-hoc test