Fig 1: Susceptibility of apical-out colon organoids to EV-A71 virus.a, Apical-out, differentiated colon epithelial organoids express the EV-A71 receptor SCARB2 (red), localizing to intracellular membranes. The integrity of the apical actin (white) microvillus brush border is visible. b, Colon organoids were infected with EV-A71. Viral titres were monitored over time by plaque assay. Data from four independent experiments using two different colonoid donors are shown. c, EV-A71-infected cells were observed by immunofluorescence assay following staining for double-stranded vRNA after 48 h of infection. Right: increased magnification of infected cell denoted by yellow arrowhead in the left panel. Scale bars, 10 μm.Source data