Fig 1: A2 deletion increases GAP-43 expression in nerve fibers after ONC. Flat mount immunolabeling (A) showed higher GAP-43 expression in nerve fibers of WT ONC vs. sham retinas at 7 days and A2 deletion further increased GAP-43 immunoreactivity. N = 4–5, scale bar = 50 µm. Western blotting on retina lysates (B) and quantification (C) showed increased GAP-43 expression in A2-/- retinas as compared to WT that was significant at 5 days but did not reach statistical significance at 7 days after ONC. N = 3, *p < 0.05 vs. WT ONC, n.s. means no statistically significant difference.