Fig 1: PRR expression and impact of HDPs on uptake and cytokine profile. The levels of (A) TLR3 by ILCs; and (B) TLR9 by total ILCs, ILC1, ILC2, and ILC3. Cell populations were normalised to 100%. Results are representative of 2 - 5 donors. (C) ILC uptake of HAdVs complexed with HNP-1 or lactoferrin: GFP expression by ILCs was quantified 24 h post-challenge with each HAdV ± HDP (HNP-1 or lactoferrin) for total ILCs (n = 4). (D) Cytokines release in the presence of HNP-1 or lactoferrin: cytokines belonging to an antiviral panel and a Th cytokine panel in the supernatant of HAdV-infected ILCs was quantified using CBA (n = 4). Cytokine levels are denoted by the colour code and the results were analyzed with the LegendPlex™ software. Only the cytokines whose secretion level was at least 2-fold higher than that of the controls are presented. (E) IL-8 secretion was assessed 24 h post-challenge with each HAdV ± HDP for total ILCs (n = 4). Statistical analyses were performed using paired Student’s t test by comparing uninfected cells and cells challenged with the HAdVs.