Fig 1: Transcriptomic analysis of the differentially expressed genes (DEGs) between CYP27A1 KO and WT osteoclasts.(A) Volcano map of the differential gene expression between CYP27A1 WT and KO groups. (B) Box plots of the differential genes between CYP27A1 WT and KO groups. (C) Heatmap of the differential gene clusters between CYP27A1 WT and KO groups. (D) The effect of CYP27A1 KO on the mRNA expression of COL1A2, BGN, SPARC, GM20708, CYP27A1, ELANE, S100A9 and LY6C2. The mRNA expression was determined using qRT-PCR. *, p < 0.05; **, p < 0.01. (E) CYP27A1 KO promoted the expression of COL1A2, BGN and SPARC. The protein expression was determined using Western blot analysis. (F) The relative expression of COL1A2, BGN and SPARC. *, p < 0.05; **, p < 0.01. (G) CYP27A1 KO inhibited the expression of ELANE, S100A9 and LY6C2. The protein expression was determined using Western blot analysis. (H) The relative expression of ELANE, S100A9 and LY6C2. *, p < 0.05; ***, p < 0.001.