Fig 1: Inhibitory effect of obacunone on abnormal cell proliferation. (A) Representative Western blots of mTOR signaling pathway proteins in the kidneys of wild-type (WT) and PKD mice (PKD) treated without (Ctrl) or with obacunone (Oba). (B) Relative phosphorylation levels of mTOR protein. (C) Relative phosphorylation levels of S6 protein. (D) Representative Western blots of MAPK signaling pathway proteins. (E) Quantification of H-Ras protein expression levels. (F) Quantification of B-Raf protein expression levels. (G) Relative phosphorylation levels of ERK protein. (H) Representative Western blots of PCNA. (I) Quantification of PCNA protein expression levels. (J) Immunofluorescence staining of Ki-67 (red) and DAPI (blue) in the PKD kidneys. Arrows represent the Ki-67 positive cells, asterisks represent the cyst area. Bar = 50 µm. (K) Quantification of Ki-67 positive cells. Data are presented as means ± SEM. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001 versus WT-Ctrl. # p < 0.05, ## p < 0.01, ### p < 0.001 versus PKD-Ctrl.