Fig 1: JNK/VEGFR2/RhoJ mediates activation of PAK and ERK pathways. (A) After HUVECs were treated with VEGF at different concentrations, cell lysates were subjected to immunoblotting to detect the activity of JNK and ERK, and (B) PAK2, PAK4. (C) The effect of SP600125 stimulation on VEGFR2 activation in HUVEC cells was detected by Western blotting. (D) Immunofluorescence staining for RhoJ, Pholloidin, and DAPI in HUVECs with or without treatment of VEGF or JNK inhibitor. Scale Bar 100 µm. (E) Quantification of fluorescence intensity of RhoJ and F-actin in (D). Scale bar, 100 µm. (F) the Cell Counting Kit-8 (CCK-8) assays of HUVECs after applying VEGF, SP600125, or vehicle. In all images, data are shown as the mean ± SEM. Every experiment was repeated for three times. *p<0.05; **p<0.01; ***p<0.001.