Fig 2: Binding disposition of docked compound 5 inside the active site of caspase-3 (A) and IFN γ (B) inhibition proteins with interactive key amino acids. Visualization was made using Chimera software [90,91].

Fig 3: Inflammatory marker (interferon gamma) and apoptotic marker (caspase 3) in the studied groups. Data are represented as mean ± SE and analyzed using ANOVA followed by Bonferroni’s post hoc test at p value < 0.05, n = 10 for each group. a indicates the presence of significant difference from normal control group, b indicates the presence of significant difference from cisplatin group.

Fig 4: (A) Level of IL-1β expression in liver and kidney tissues of the experimental groups. Positive proportions of IL-1β expression were increased significantly in liver and kidney of cisplatin treated rats compared to the normal control and C. annua pretreated group. (B) Level of caspase-3 expression in liver and kidney tissues of the experimental groups. Positive proportions of caspase-3 expression were elevated remarkably in liver and kidney of cisplatin treated rats compared to the normal control and C. annua pretreated group. Data are represented as mean ± SE and analyzed using ANOVA followed by Bonferroni’s post-hoc test at p value < 0.05, n = 15 for each group. a indicates the presence of significant difference from normal control group, b indicates the presence of significant difference from cisplatin group.