Fig 2: Immunohistochemical staining against PSD-95. PSD-95 (green) in frozen sections of the retina of the mouse eye at 24 weeks and 55 weeks of control and Nrg1-III-tg as indicated. The cell nuclei were stained with DAPI (blue); GCL-ganglion cell layer; IPL-inner plexiform layer; INL-inner nuclear layer; OPL-outer plexiform layer; ONL-outer nuclear layer. Scale bar: 50 µm. ** p < 0.01.

Fig 3: The Volume of retina in 12-, 24- and 55-week-old mice. Data are shown as mean values. Error bars show standard error of means. Number of animals at the age of 12, 24 and 55 weeks: n = 12, n = 13 and n = 14 control mice and n = 16, n = 8 and n = 10 Nrg1-III-tg age-matched mice, respectively.

Fig 4: Immunohistochemical staining against neuregulin. The trophic factor neuregulin (red) in frozen sections of the retina of the mouse eye at 24 weeks and 55 weeks of control and Nrg1-III-tg as indicated. The cell nuclei were stained with DAPI (blue); GCL-ganglion cell layer; IPL-inner plexiform layer; INL-inner nuclear layer; OPL-outer plexiform layer; ONL-outer nuclear layer. Scale bar: 50 µm.

Fig 5: ERG Parameters in 12-, 24 and 55-week control and transgenic mice. Scotopic amplitudes (A) and latencies (B) of a-waves and b-waves at different light intensities as indicated: (C): amplitudes and latencies of scotopic oscillatory potentials; (D): the ratio of amplitudes of b-waves and a-waves. Number of animals at the age of 12, 24 and 55 weeks: n = 8, n = 13 and n = 9 control mice and n = 6, n = 5 and n = 8 Nrg1-III-tg age-matched mice, respectively. Data are shown as mean values. Error bars show standard error of means. Statistical significance of differences is indicated as follows: * p < 0.05, *** p < 0.001.