Fig 1: Representative western blot and densitometric analysis showing level of PGC1α (a), MFN1 (b), MFN2 (c), OPA1 long form (L-OPA1, 95kD) and short form (S-OPA1, 75kD) (d), and DRP1 (e) proteins in RMC cells following 0, 30, and 60 μM of bicalutamide treatment for 24 and 48 h. β-Actin is as cytosolic normalization control; HSP60 is as mitochondrial normalization control. Bar graph represents the mean ± SD of triplicate experiments: * p < 0.05, ** p < 0.01 *** p < 0.001 compared to Bic 0 μM at 24 h. ## p < 0.01 compared to Bic 30 μM at 24 h. § p < 0.05, §§ p< 0.01, §§§ p < 0.001 compared to Bic 0 μM at 48 h. p < 0.001 compared to Bic 30 μM at 48 h.