Fig 1: The RAF-MEK-ERK pathway regulates the sorting of HCV into APPL1 and EEA1-associated early endosomes.(A, C, E, and G) Huh-7.5 spheroids were incubated with DMSO or 5 μM sorafenib for 2 hr, infected with DiD-HCV (red) with DMSO or sorafenib for 1 hr at 4°C, shifted to 37°C for the indicated times, fixed, and probed for CLDN1 (A), Rab5 (C), APPL1 (E) or EEA1 (G) (green). (B, D, F, and H) Quantitation of (A), (C), (E) and (G), respectively. n = total DiD signal. Mean +/- SEM. *p < 0.05, ***p < 0.001.