Fig 1: The wild-type CD24+CD29lo Sca1+ CD133+ LCs are required for paracrine signaling to restore the regenerative potential of basal cells. (A) Representative gating strategy illustrating LCs (CD24+ CD29lo) being subgated for Sca1+ CD133+ and Sca1− CD133− subpopulations. (B) Relative quantities of Esr1, Pgr and Areg RNA normalized to B2m, Hprt, Gusb and Tbp in wild-type Sca1+CD133+ LCs when compared with wild-type Sca1−CD133− LCs (**P<0.01, ***P<0.001; Mann–Whitney test). (C) Representative images of GFP-positive outgrowths arising from the transplantation of C451A-ERα MACS with sorted Sca1+ CD133+ or Sca1− CD133− wild-type LCs. Scale bars: 2 mm. (D) Confocal images of mammary gland epithelium after immunostaining with anti-GFP (green), anti-K5 (red) and anti-K8 (magenta) primary antibodies. Scale bars: 20 µm. (E) Confocal images of mammary gland epithelium after immunostaining using anti-GFP (green) and anti ERα (red) antibodies. Scale bar: 20 µm.