Fig 2: Western blots (WB) and quantitative analysis of an independent set of the urinary exosomes isolated from control and 5XFAD mice. (A) Same aliquot of urinary exosomes isolated from six control samples and six 5XFAD mice samples. AOAH, NEU1, Annexin 2, Clusterin, and Ly86 proteins were further detected by WB in sequence. Alix and Flotillin 1 were tested as urinary exosomes biomarkers. Representative blots of the tested proteins and the molecular weight of standard proteins were presented. (B–F) Quantitative analysis of each of the tested protein’s relative fold change, respectively. All data are presented as mean ± SD in triplicate experiments; Student’s t-test; *P < 0.05; **P < 0.01; ***P < 0.001.