Fig 1: MiR-379-5p re-expression elevates DDP-resistant lung cancer cell sensitivity to DDP via targeting E2F7. (a–i) A549/DDP and H1299/DDP cells were transfected with miR-NC, miR-379-5p, miR-379-5p + pcDNA, or E2F7. (a) Western blot analysis of E2F7 protein levels in A549/DDP and H1299/DDP cells. (b) Cell counting kit-8 (CCK-8) assay of the IC50 values of A549/DDP and H1299/DDP cells to DDP. The proliferation (c, d), apoptosis (e), invasion (f) and migration (g) of transfected A549/DDP and H1299/DDP cells under 25 µM DDP treatment were determined using cell counting kit-8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridine (EdU), flow cytometry, transwell, and wound healing assays, respectively. (h, i) Western blot analysis of cyclin D1, MMP9, and MDR1 protein levels in A549/DDP and H1299/DDP cells under 25 µM DDP treatment. ***p < 0.001 and ****p < 0.0001.
Fig 2: Circ_0010235/miR‐379‐5p axis can regulate E2F7 expression. (a, b) Western blot analysis of E2F7 expression in A549/DDP and H1299/DDP cells transfected with si‐NC, si‐circ_0010235, si‐circ_0010235 + anti‐miR‐NC or si‐circ_0010235 + anti‐miR‐379‐5p. **p < 0.01, ***p < 0.001, ****p < 0.0001.
Fig 3: E2F7 is a target of miR‐379‐5p. (a) The binding sites between miR‐379‐5p and E2F7 are shown. (b, c) Dual‐luciferase reporter assay for the luciferase activity of wild and mutated E2F7 reporter after miR‐379‐5p overexpression in A549/DDP and H1299/DDP cells. (d) Quantitative reverse transcription PCR (qRT‐PCR) analysis of E2F7 mRNA expression in 27 DDP‐sensitive and 28 DDP‐resistant lung cancer tissues. (e) E2F7 mRNA expression was negatively correlated with miR‐379‐5p expression in DDP‐resistant lung cancer tissues. (f) Western blot analysis of E2F7 protein levels in 27 DDP‐sensitive and 28 DDP‐resistant lung cancer tissues. (g) Western blot analysis of E2F7 protein levels in normal HBE, A549, H1299, A549/DDP and H1299/DDP cells. (h) The interference efficiency of miR‐379‐5p inhibitor or inhibitor NC in A549/DDP and H1299/DDP cells using qRT‐PCR. (i) Western blot analysis of E2F7 protein levels in miR‐379‐5p up‐ or downregulated A549/DDP and H1299/DDP cells. ***p < 0.001 and ****p < 0.0001.
Supplier Page from Thermo Fisher Scientific for E2F7 Antibody