Fig 1: Severe interlobular bile ducts injury, fibrosis, and CD20 cells infiltration were observed in pre-sensitized followed by transplant rats. (A) Interlobular bile ducts were marked by CK19 in each group by IHC staining. (B) Degree of fibrosis was detected by Masson staining. (C) CD20 B cells were marked in each group by IHC staining. LEW, Lewis; IHC, immunohistochemistry.
Fig 2: Uncovering HSC Zonation across the Healthy Liver Lobule(A) Heatmap of relative expression (center): cubic smoothing spline curves fitted to markers of HSC zonation and plotted along IC2; genes are thresholded and ordered on their contribution to IC2, with top-most genes displaying the strongest negative correlation with bottom-most genes. Cells columns, genes rows. Zonation profiles for exemplar genes shown left and right.(B) Representative immunofluorescence and RNAscope images of healthy murine livers: NGFR/Adamtsl2 (RNAscope) (red), E-cadherin/Cyp2e1 (green), DAPI (blue). Scale bar, 100 µm. Yellow arrows indicate Adamtsl2+ cells. ICA visualizations (below): Ngfr and Adamtsl2 expression on the first and second independent components of the HSC subpopulation in homeostatic murine liver. Bar plots (below): number of PaHSCs (left; n = 4) and CaHSCs (right; n = 4) per mm2 in peri-portal and peri-central regions; error bars SEM, Mann-Whitney test, *p < 0.05.(C) Schematic representation of the topography of the two HSC subpopulations in healthy liver lobule. CV, central vein; PV, portal vein; HA, hepatic artery; BD, bile duct.(D) Representative immunofluorescence images of healthy human livers: NGFR/ADAMTSL2 (red), CK19 (biliary epithelial cell marker; green), DAPI (blue). Scale bar, 100 µm; portal vein (*) and central vein (#) as indicated. Yellow dashed lines mark areas of low/neg marker staining.See also Figures S4 and S5.
Supplier Page from Abcam for Anti-Cytokeratin 19 antibody [KRT19/800]