Fig 1: Double-immunostained labeling of KIF5B and GAP43 protein expression in the rat gastrocnemius muscle. (a) Representative image of KIF5B and GAP43 co-localization analysis showing GAP-43 (green), KIF5B (red) and myonuclei (blue). (b 1–3) Higher magnification of selected area (yellow box) in image (a). (c) GAP-43-KIF5B positive Cells (% GAP-43+) in different experimental groups. White bar = 50 µm. **p < 0.001. Statistical analysis was performed using the Graph-Pad Prism statistics software (Graph-Pad Software Inc., San Diego, CA, USA free demo version 5.04).
Fig 2: Kif5B and GAP-43 expression in different experimental groups. (a) Representative image of Kif5B analysis. (e) Number of Kif5B+/100 fibers. (c) Correlation between Kif5B+/100 fibers and the number of nuclei per fiber. (d 1–4) Higher magnification of selected area (yellow box) in image (a) showing Laminin (red), Kif5B (green) and myonuclei (blue). (e) Correlation between Kif5B+/100 fibers and average gastrocnemius muscle CSA. (f) Representative image of GAP-43 analysis. (g) Number of GAP-43+/100 fibers. (h) Correlation between GAP-43+/100 fibers and the number of nuclei per fiber. (i 1–4) Higher magnification of selected area (yellow box) in image (f) showing Laminin (green), GAP-43 (red) and myonuclei (blue). (j) Correlation between GAP-43+/100 fibers and average gastrocnemius muscle CSA. (k) Representative Western blot images from different experimental groups. Expression of Kif5B (l), and GAP-43 (m) further demonstrated by Western blotting normalized to GAPDH. White bar = 50 µm. **p < 0.001. Full gel-images are available in Supplementary Material. Statistical analysis was performed using the Graph-Pad Prism statistics software (Graph-Pad Software Inc., San Diego, CA, USA free demo version 5.04).
Supplier Page from Abcam for Anti-GAP43 antibody [EP890Y] - BSA and Azide free