Fig 1: Administration of Fn1 inhibitor increased the AMP/ATP ratio and activated LKB1-AMPK signaling in I/R hearts. (a) Immunoblotting analysis of the protein levels of p-LKB1 (S428), LKB1, p-AMKP (T172), AS160, GLUT4, Mfn1, Mfn2, (left), p-Drp1 (S616), and Drp1. GAPDH as an internal control. (b) The quantification of the relative protein levels (right, n = 4 per group). (c) Measurement of AMP and ATP contents and the ratio of AMP/ATP (n = 5). Data are expressed as mean ± SEM. ***P < 0.001; ****P < 0.0001 versus the sham-operated group; ##P < 0.01; ###P < 0.001, ####P < 0.0001 versus saline I/R 24 h; &&P < 0.01; &&&P < 0.001, &&&&P < 0.0001 versus saline I/R 72 h.