Purified Mouse IgG1, kappa Isotype Ctrl 401401 from BioLegend

Product Specs
ItemPurified Mouse IgG1, kappa Isotype Ctrl
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number401401
CloneMG1-45
HostMouse
Conjugate/TagUnconjugated
Quantity50 µg
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
IsotypeIgG1, Kappa
ApplicationsFlow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), Chromatin Immunoprecipitation
Concentration0.50 mg/mL
ImmunogenTrinitrophenol + KLH
ClonalityMonoclonal

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BioLegend

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Website: biolegend.com

(1) MICa/b‐dependent activation of natural killer cells by CD64 + inflammatory type 2 dendritic cells contributes to autoimmunityThe EMBO JournalDecember 1, 2023Ildefonso Sánchez‐Cerrillo, Diego Calzada‐Fraile, Ana Triguero‐Martínez, Marta Calvet‐Mirabent, Olga Popova, Cristina Delgado‐Arévalo, Mariel Valdivia‐Mazeyra, Marta Ramírez‐Huesca, Enrique Vázquez de Luis, Alberto Benguría, et al.anti‐IL‐15 (4 μg/ml/MAB2471/R&D Systems) or their corresponding isotypic controls (4 μg/ml/402202 and 401402/BioLegend) Abs were added to the cultures to address the functional relevance of NKG2D‐ligand and IL‐15‐receptor

(2) VE-cadherin in arachnoid and pia mater cells serves as a suitable landmark for in vivo imaging of CNS immune surveillance and inflammationNature communicationsSeptember 20, 2023Mapunda JA, Pareja J, Vladymyrov M, Bouillet E, Hélie P, Pleskač P, Barcos S, Andrae J, Vestweber D, McDonald DM, et al.esculentum (tomato) lectin-DyLight 594 (Invitrogen, Cat No L32471), mouse IgG1 κ isotype (BioLegend, Cat No 401402), rabbit IgG (R&D, Cat No AB-105-C), goat IgG (R&D, Cat No AB-108-C). Secondary antibodies used in this

(3) Understanding the heterogeneity of alloreactive natural killer cell function in kidney transplantationbioRxivSeptember 12, 2023Dan Fu Ruan, Miguel Fribourg, Yuko Yuki, Yeon-Hwa Park, Maureen Martin, Geoffrey Kelly, Brian Lee, Ronaldo Miguel Miguel de Real, Rachel Lee, Daniel Geanon, et al.anti-DNAM-1-146Nd (Life Technologies, cat #MA5–28149) or mouse IgG1 k isotype control (BioLegend, cat #401402) for 30 minutes at room temperature prior to addition of stimulator cells. Assays were ended by placing

(4) Quantification of membrane-bound cytokine receptors by calibrated flow cytometrySTAR ProtocAugust 14, 2023Niloufarsadat Miri, Nadine Köhler, Anna Dittrich(1:50)",Diaclone,Cat# 852.060.000 Mouse IgG Isotype Control (RRID: AB_2801452) (1:25),BioLegend,Cat# 401401 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(5) Phenotypic diversity of T cells in human primary and metastatic brain tumors revealed by multiomic interrogationNature CancerMay 22, 2023Vladimir Wischnewski, Roeltje R. Maas, Paola Guerrero Aruffo, Klara Soukup, Giovanni Galletti, Mara Kornete, Sabine Galland, Nadine Fournier, Johanna Lilja, Pratyaksha Wirapati, et al.µg ml–1 of the blocking anti-PD1 (clone EH12.2H7, BioLegend, 329926) or isotype control (BioLegend, 401401) was added. The cells were incubated at 37 °C and 5% CO2 for 96 h. Afterward, cells were stained with

(6) C4b-binding protein inhibits particulate- and crystalline-induced NLRP3 inflammasome activationFrontiers in ImmunologyMay 22, 2023Damien Bierschenk, Nikolina Papac-Milicevic, Ian P. Bresch, Valentina Kovacic, Serena Bettoni, Mateusz Dziedzic, Rick A. Wetsel, Susanne Eschenburg, Christoph J. Binder, Anna M. Blom, et al.subtype, Normal Rabbit IgG (Merck Millipore, 12-370) and mouse IgG1, κ (Biolegend, San Diego, CA, USA, 401401), at the same concentration as primary antibodies used in the PLA. Lysosomal galectin-3 staining HMDM

(7) Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro StudyInternational Journal of Molecular SciencesApril 19, 2023Hussein M. El-Husseiny, Masahiro Kaneda, Eman A. Mady, Tadashi Yoshida, Ahmed S. Doghish, Ryou TanakaUSA)","Goat anti-mouse IgG H&L (Alexa Fluor® 488)(ab150113, Abcam, Cambridge, UK)","Purified Mouse IgG1, κ(401402, BioLegend, San Diego, CA, USA)" Specifics of antibodies employed for flow cytometric analysis.

(8) CD161 Characterizes an Inflamed Subset of Cytotoxic T Lymphocytes Associated with Prolonged Survival in Human Papillomavirus–Driven Oropharyngeal CancerCancer Immunology ResearchMarch 1, 2023Ye Wei, Tingting Xu, Chong Li, Xin Zhou, Wei Qian, Chunying Shen, Qifeng Wang, Xing Xing, Xiaomin Ou, Xiayun He, et al.mAb (BioLegend, clone HP-3G10, cat. #339902, 10 μg/mL) or its IgG isotype control (BioLegend, cat. #401402, 10 μg/mL) for 72 hours and labeled with a secondary fluorescent antibody rat anti-mouse IgG1 PE-Cyanine7

(9) CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes ChemoresistanceCancer immunology researchMarch 1, 2023Lao L, Zeng W, Huang P, Chen H, Jia Z, Wang P, Huang D, Chen J, Nie Y, Yang L, et al.For some experiments, CD161-blocking mAb (BioLegend, #339902) or mouse IgG control Ab (BioLegend, # 401402) was added to the coculture at a final concentration of 10 μg/mL. The OD values were measured with a

(10) Optimal temperature for the long-term culture of adult porcine islets for xenotransplantationFrontiers in immunologyJanuary 1, 2023Sakata N, Yoshimatsu G, Kawakami R, Aoyagi C, Kodama S.(Cat#401601; RRID : AB_2935847; 1:50; BioLegend, San Diego, CA, USA), purified mouse IgG1, κ Isotype Ctrl (Cat#401402, RRID : AB_2801451; 1:5; BioLegend), or purified rabbit polyclonal Isotype Ctrl (Cat#910801, RRID :

(11) Use of poxvirus display to select antibodies specific for complex membrane antigensmAbsJanuary 1, 2023Smith ES, Balch LA, Scrivens M, Shi S, Wang W, Harvey CD, Cornelison AA, Gil-Moore M, Kirk RA, Mueller LL, et al.purchased from BD Biosciences. Mouse Isotype controls were purchased from Biolegend (mouse IgG1,K cat# 401402 Biolegend; mouse IgG2a,K cat #401508 Biolegend), and Human IgG Isotype control was purchased from Southern

(12) Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assaySTAR protocolsDecember 16, 2022Nasser H, Shimizu R, Ito J, Genotype to Phenotype Japan (G2P-Japan) Consortium, Saito A, Sato K, Ikeda T.0111-01; RRID: AB_2732899 Normal mouse IgG (clone MG1-45) (1:100 for FACS analysis),BioLegend,Cat# 401401;RRID: AB_2801452 APC-conjugated goat anti-rabbit IgG polyclonal antibody (1:50 for FACS analysis),Jackson ImmunoResearch

(13) Platelet-Neutrophil Association in NETs-Rich Areas in the Retrieved AIS Patient ThrombiInternational journal of molecular sciencesNovember 21, 2022Pir GJ, Parray A, Ayadathil R, Pananchikkal SV, Mir FA, Muhammad I, Abubakar A, Amir N, Hussain S, Haroon KH, et al.antibody (910801; Biolegend, San Diego, CA 92121, USA) or monoclonal mouse IgG1κ isotype control antibody (401402, Biolegend). 4.2. Immunofluorescence Staining For Immunofluorescence, deparaffinized thrombi sections

(14) IL-2â€“mTORC1 signaling coordinates the STAT1/T-bet axis to ensure Th1 cell differentiation and anti-bacterial immune response in fishPLOS PathogensOctober 25, 2022Kete Ai, Kang Li, Xinying Jiao, Yu Zhang, Jiaqi Li, Qian Zhang, Xiumei Wei, Jialong YangTilapia individuals were i.p. injected with 20 μg of mouse anti-tilapia CD4-1 or mouse IgG1 (Catalog #401402, BioLegend) as isotype control antibody. After 24 h, the tilapia was i.p. injected with 50 μL tilapia

(15) Tumor targeted 4-1BB agonist antibody-albumin fusions with high affinity to FcRn induce anti-tumor immunity without toxicityiScienceSeptember 16, 2022Hangiu O, Compte M, Dinesen A, Navarro R, Tapia-Galisteo A, Mandrup OA, Erce-Llamazares A, Lázaro-Gorines R, Nehme-Álvarez D, Domínguez-Alonso C, et al.chimera,SinoBiological,Cat#50811-M02H Biotinylated human FcRn,Immunitrack,Cat#ITF01 Mouse IgG1K,Biolegend,Cat#401402 FcRn wild-type-binding albumin,Sigma-Aldrich,Cat#A6608 Streptavidin,Sartorius,Cat#18-5019 ,, Critical

(16) Greatly Enhanced CTC Culture Enabled by Capturing CTC Heterogeneity Using a PEGylated PDMS-Titanium-Gold Electro-Microfluidic Device with Glutathione-Controlled Gentle Cell ReleaseACS NanoJuly 26, 2022Elyahb A. Kwizera, Wenquan Ou, Sojeong Lee, Samantha Stewart, James G. Shamul, Jiangsheng Xu, Nancy Tait, Katherine H. R. Tkaczuk, Xiaoming HeALDH1A1 antibody (clone M562, Cat # 861902), Purified mouse isotype control IgG1 (clone MG1–45, cat # 401402), Purified anti-mouse CD24 Antibody (clone 30-F1, Cat: 138502) monoclonal antibodies were purchased

(17) Active PD-L1 incorporation within HIV virions functionally impairs T follicular helper cellsPLoS pathogensJuly 1, 2022Munoz O, Banga R, Schelling R, Procopio FA, Mastrangelo A, Nortier P, Ohmiti K, Daraspe J, Cavassini M, Fenwick C, et al.conjugated with either anti-PD-L1 mAbs (MIH1, Invitrogen; #14-5983-82) or an isotype-control (Biolegend; #401402) using the Functional Bead Conjugation Buffer Set protocol (BD; #558556) following the manufacturer’s

(18) Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communicationImmunityJune 14, 2022Gargaro M, Scalisi G, Manni G, Briseño CG, Bagadia P, Durai V, Theisen DJ, Kim S, Castelli M, Xu CA, et al.RTK2758)",BioLegend,Cat#: 400502; RRID: AB_326523 Mouse IgG1k Isotype Ctrl (clone: MG1-45),BioLegend,Cat#: 401402; RRID: AB_2801451 Biotin or APC human anti-human CD141(clone: REA674),Milteny Biotech,"Cat#: 130-114-186,

(19) Cytokine-enhanced cytolytic activity of exosomes from NK CellsCancer Gene TherapyJune 1, 2022Yutaka Enomoto, Peng Li, Lisa M. Jenkins, Dimitrios Anastasakis, Gaelyn C. Lyons, Markus Hafner, Warren J. Leonardexperiments with blocking antibodies to CD226, NK-EVs were incubated with isotype control (Biolegend, clone 401402) or anti-CD226 antibodies (Abcam, clone DX11) [30, 31] for 30 min at 37 °C and then incubated with K562 cells

(20) Platelets Fuel the Inflammasome Activation of Innate Immune CellsSSRN Electronic JournalApril 1, 2022Verena Rolfes et al.CD42b Antibody (HIP1) BioLegend 303902; RRID:AB_314382 Purified Mouse IgG, k Isotype BioLegend 401402; RRID:AB_2801451 Rat Anti-mouse CD14-FITC (Sa14-2) BioLegend 123308; RRID:AB_940580 Rat IgG1 K Iso

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12/21/2018IgG1 Control Jamie WallisThis control provides an excellent standard when quantifying total IgG1 in murine serum samples.

Purified Mouse IgG1, kappa Isotype Ctrl from BioLegend

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Purified Mouse IgG1, kappa Isotype Ctrl from BioLegend

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