4-Hydroxynonenal Antibody (12F7)

Western Blot: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353] - Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7 (NBP2-59353). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: 4-hydroxyl nonenal-BSA (0.5 ug). Lane 4: 4-hydroxy nonenal-BSA (2.0 ug). Lane 5: 4-hydroxy-2-hexenal (0.5 ug). Lane 6: 4-hydroxy-2-hexenal (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody (NBP2-59353) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Western Blot: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353] - Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7 (NBP2-59353). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: 4-hydroxyl nonenal-BSA (0.5 ug). Lane 4: 4-hydroxy nonenal-BSA (2.0 ug). Lane 5: 4-hydroxy-2-hexenal (0.5 ug). Lane 6: 4-hydroxy-2-hexenal (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody (NBP2-59353) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.