Fig 1: Characteristics of mouse E-MNCs. (A) Phase-contrast imaging of CB6F1 mouse PBMNCs (at day 0) and E-MNCs (at day 7). White boxed areas in the upper images (scale bar, 100 µm) were magnified in the lower images (scale bar, 100 µm). (B) Flow cytometric analysis of FSC/SSC gated cells, CD11b+/CD206− (Mono-naïve) and CD11b+/CD206+ (M2) macrophages, among PBMNCs (at day 0) and E-MNCs (at day 7). (C) Percentages of Mono-naïve (CD11b+/CD206−) and M2 (CD11b+/CD206+) macrophage fractions and their ratios (Mono-naïve/M2) among PBMNCs and E-MNCs (*p < 0.05). (D) Flow cytometric analysis of CD11b+/Msr1+ (M2c; left panels) macrophages and CCR2/galectin3+ (right panels) CD11b-positive macrophages among PBMNCs (at day 0) and E-MNCs (at day 7). (E) Flow cytometric analysis of CCR6−/CCR4+ Th2 cells in CD3+/CD4+ gated cells of PBMNCs (at day 0) and E-MNCs (at day 7). (F) Expression of pro-inflammatory mRNAs (il-1β, ifn-γ, and tnf-α) in PBMNCs (at day 0) and E-MNCs (at day 7) (*p < 0.05, **p < 0.01). For statistical analysis, the Student’s t-test was performed to determine the significance of differences among PBMNCs and E-MNCs.